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Saturday, July 27, 2013

How to Create Your Own BLAST protein database and use BLAST in Windows

Hi, so this is my second post about Science in this blog. I actually have a lot in mind to write in here but i have been extremely busy doing my research. Anyway, what i want to share in here is actually very simple, however, for such a beginner in protein sequence like me it should be a useful post. So here is the thing, i want to find homologous pair of a protein, BLAST particularly blastp can generate and score some proteins that potentially homologous to another protein. Let's start. 1st step --- Download the latest BLAST from NCBI site here. In my case i used BLAST Win-32. Just follow the instructions when installing, then it should be installed in C:\Program Files\NCBI\blast-2.2.28+. 2nd step --- Prepare your FASTA sequence file. In my case i tried to find homologous pair...

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