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Wednesday, August 7, 2013

Create Boxplot with legend from text file and coloring them in R

So i have been playing around with R today (this is my very first time using it), a statistical tool and i wanted to draw a boxplot analysis for my research. I am not going to explain what boxplot is here (you may find some resources explaining about it through the internet but here's one in Wikipedia), but more to how to create it in R. So if you have R already installed on your machine let's start it. First of all, i used R Commander (Rcmdr) to import my txt file to R dataset. 1st step ---- If you have Rcmdr package already, you may skip to '2nd step ----'. But if you don't, following are the instructions. > Go to Packages > Install...

Saturday, July 27, 2013

How to Create Your Own BLAST protein database and use BLAST in Windows

Hi, so this is my second post about Science in this blog. I actually have a lot in mind to write in here but i have been extremely busy doing my research. Anyway, what i want to share in here is actually very simple, however, for such a beginner in protein sequence like me it should be a useful post. So here is the thing, i want to find homologous pair of a protein, BLAST particularly blastp can generate and score some proteins that potentially homologous to another protein. Let's start. 1st step --- Download the latest BLAST from NCBI site here. In my case i used BLAST Win-32. Just follow the instructions when installing, then it should be installed in C:\Program Files\NCBI\blast-2.2.28+. 2nd step --- Prepare your FASTA sequence file. In my case i tried to find homologous pair...

Tuesday, August 7, 2012

Autodock Tools (ADT) and Python Imaging Library (PIL) Installation on Ubuntu 32-bit

I was struggled installing this Autodock Tools on Ubuntu for days, because of the Python Imaging Library issues ("decoder jpeg not available|" and "decoder zip not available"). Ubuntu and all its stuff is actually quiet new to me since i've been working on Windows for ages. Anyway, i wrote down the steps to solve my issues here: 1st step--- Before you're moving too far to install MGLTools, first it's better to figure out your Python Imaging Library (PIL) installation. If you have installed PIL then you need to : sudo pip uninstall PIL sudo apt-get install build-essential python-dev zlib1g-dev liblcms1-dev libjpeg8 libjpeg62-dev libfreetype6 libfreetype6-dev sudo ln -s /usr/lib/i386-linux-gnu/libfreetype.so /usr/lib sudo ln -s /usr/lib/i386-linux-gnu/libz.so /usr/lib sudo ln -s...

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